Publisher: John Wiley & Sons Inc
E-ISSN: 1439-7633|16|12|1803-1810
ISSN: 1439-4227
Source: CHEMBIOCHEM, Vol.16, Iss.12, 2015-08, pp. : 1803-1810
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
AbstractThe RNA cleavage activity of the hammerhead ribozyme has been compared in various mixed aqueous solutions containing cosolvents. Kinetic analysis revealed that the tested cosolvents enhanced the ribozyme activity, particularly at low MgCl2 concentrations. These enhancements, in some cases of more than tenfold, resulted from a reduction in the Mg2+ concentration required for substrate cleavage. An inverse correlation was found between the MgCl2 concentration essential for efficient catalysis and the dielectric constant values. In contrast, FRET measurements showed no substantial influence of cosolvents on the Mg2+‐induced structural transitions. The results suggest that the solution environment has various effects on the Mg2+ interactions involved in the catalysis and global folding of the ribozyme.
Related content
Modulation of Ribozyme and Deoxyribozyme Activities Using Tetraalkylammonium Ions
CHEMPHYSCHEM, Vol. 18, Iss. 24, 2017-12 ,pp. :
By Sahoo Gobinda Prasad Basu Samita Samanta Sadhan Misra Ajay
Journal of Experimental Nanoscience, Vol. 10, Iss. 9, 2015-06 ,pp. :