Cover

Half-title

Title

Copyright

Contents

List of contributors

Preface

Non-radioisotopic labels for in situ hybridisation histochemistry: a histochemist's view.

Introduction

Theoretical background

Probe production

Probe labelling methods

Non-radioactive labelling systems

In situ hybridisation protocol

Hybridisation

Applications of non-radioisotopic in situ hybridisation

Future developments

References

Use of haptenised nucleic acid probes in fluorescent in situ hybridization

General

Haptenisation of probes

The in situ hybridisation procedure

Fluorescent reporter molecules

Applications

References

The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system

Introduction

Methodology

Expression of peptide mRNAs in the neuroendocrine system

Conclusions

Protocols

References

Contributions of the spatial analysis of gene expression to the study of sea urchin development

Early motivations

Methodology

Commitments to specific patterns of gene expression in the early sea urchin blastula

Tissue-specific messages in the very early blastula and early synthesis of tissue-specific nuclear proteins

Determination and differentiation of the ectoderm

Concluding observations on patterns of gene expression in the sea urchin embryo

Acknowledgments

References

Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development

Introduction

Methodology

The genetic analysis of Drosophila development: an historical perspective

Identification and analysis of embryonic lethal mutations

In situ analysis of segmentation gene expression

Transcription does not necessarily imply function

The redeployment of segmentation gene transcripts reveals novel functions for their products

Conclusions

References

The use of in situ hybridisation to study the localisation of maternal mRNAs during Xenopus oogenesis

Introduction

Results

Discussion

Materials and methods

References

In situ hybridisation in the analysis of genes with potential roles in mouse embryogenesis

Introduction

Results

Discussion

Protocols

References

Evolution of algal plastids from eukaryotic endosymbionts

Introduction

Cryptomonads

Chlorarachnion

Discussion

Materials and methods

Acknowledgements

References

Localisation of expression of male flower-specific genes from maize by in situ hybridization

Introduction

Isolation and characterisation of MFS cDNAs

In situ hybridisation with maize MFS cDNAs

Conclusions

Protocols

Acknowledgements

References

Tissue preparation techniques for in situ hybridisation studies of storage-protein gene expression during pea seed development

Introduction

Early embryo development

Cotyledonary storage deposition

Comparison of methods

Conclusions

Protocols

References

Investigation of gene expression during plant gametogenesis by in situ hybridization

Introduction

The problems posed by plant reproductive systems

The development of non-isotopic high resolution in situ systems for plant reproductive cells

Quantification of in situ hybridisation

In situ nick translation in plant reproductive cells

Acknowledgements

References

Sexing the human conceptus by in situ hybridization

Introduction

Prenatal diagnosis

Sexing by DNA-DNA in situ hybridisation

Sexing the fetus

Sexing the pre-embryo: a possible test for preimplantation diagnosis

Identification of Y-bearing spermatozoa

Polyploid nuclei

Future use of in situ hybridisation for prenatal diagnosis

Protocols

Acknowledgements

References

Non-isotopic in situ hybridisation in human pathology

Introduction

Endogenous nuclear DNA: interphase cytogenetics

Exogenous nuclear DNA

Endogenous cytoplasmic RNA

Exogenous RNA

Analysis of human disease

Conclusion

Acknowledgements

References

The demonstration of viral DNA in human tissues by in situ DNA hybridization

Introduction

Results

Methodology

Protocols

References

Index