Transcriptome (ESTs) of Avocado “Native” Mexicano Early Seed Development Shows Abundance of Regulatory, Antioxidant and Defense Genes ( Advances in Seed Biology )

Publication series : Advances in Seed Biology

Author: Luis María Suárez-Rodríguez Fernando Sánchez-Albarrán HuberLeón-Corona and Rodolfo López-Gómez  

Publisher: IntechOpen‎

Publication year: 2017

E-ISBN: INT6817870573

P-ISBN(Paperback): 9789535136217

P-ISBN(Hardback):  9789535136224

Subject: TS2 food industry

Keyword: 食品工业

Language: ENG

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Transcriptome (ESTs) of Avocado “Native” Mexicano Early Seed Development Shows Abundance of Regulatory, Antioxidant and Defense Genes

Description

Avocado native “Mexicano” (Persea americana var drymifolia) has been a really important species in agricultural and indigenous medicine. In the agricultural world, it has been the germplasm source for the generation of economically important cultivars like Hass and it is the main source of rootstocks for the world production of Hass avocado fruit. In spite of its importance, little is known about the molecular network of seed-fruit development. The aim of this work was to know the expressed genes (ESTs) during the early avocado native “Mexicano” seed development. Using total RNA we constructed cDNA libraries of fourth months seed development, sequencing, assembling and bioinformatic analysis was made. For validation, a semi-quantitative PCR experiments with the most abundant genes were made. About 5005 ESTs from the 5’ representing 1653 possible unigenes were isolated. After assembling process, we have 171 genes that are closely related to Nelumbo nucifera sequences. The transcriptome is dominating by one bHLH transcription factor, three metallothioneins and snakin, suggesting its main role in seed development. Until now, there are no molecular studies in avocado seed development.

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