Negative and Positive Staining in Transmission Electron Microscopy for Virus Diagnosis
( Microbiology in Agriculture and Human Health )
Publication series ： Microbiology in Agriculture and Human Health
Author： Debora Ferreira Barreto-Vieira and Ortrud Monika Barth
Publisher： IntechOpen
Publication year： 2015
E-ISBN: INT5964560511
P-ISBN(Paperback): 9789535121442
P-ISBN(Hardback): 9789535121442
Subject： Q93 Microbiology
Keyword： 微生物学
Language： ENG
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Negative and Positive Staining in Transmission Electron Microscopy for Virus Diagnosis
Description
Visualization of virus particles and morphological features depends on the resolution of microscopes. Transmission electron microscopy (TEM) is the starting point for obtaining the best resolution of images. Two different techniques are available and described in this paper. Firstly, negative staining of viral suspensions provides detailed information of virus particles' structure. It is a technique that can be quickly performed and is able to accommodate the highest magnifications of virus particles. Secondly, ultra-thin sections of virus-infected tissues or cell cultures, combined with a positive staining technique can provide information regarding the localization of viruses inside or around cells. These two complementary techniques for investigating the structure of a virus and its parasitic life cycle are presented in this paper.
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