Cryopreservation of Adherent Cells on a Fixed Substrate ( Recent Advances in Cryopreservation )

Publication series ： Recent Advances in Cryopreservation

Author： Lia H. Campbell and Kelvin G.M. Brockbank

Publisher： IntechOpen‎

Publication year： 2014

E-ISBN: INT5699858618

P-ISBN(Paperback): 9789535116448

P-ISBN(Hardback): 9789535116448

Subject： Q93 Microbiology

Keyword：微生物学

Language： ENG

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Cryopreservation of Adherent Cells on a Fixed Substrate

Chapter

Chapter 1 Cryopreservation of Adherent Cells on a Fixed Substrate

1. Introduction

2. Materials and methods

3. Results

3.1. Initial protocol setup

3.2. Warming rates

3.3. Cooling conditions

3.4. Cryopreservation in bulk solution vs. air

3.5. Exogenous nucleating agents

3.6. Effects on cell survival

4. Other parameters

4.1. Optimized system

4.2. Carrier solution

4.3. Apoptosis

4.4. Well configurations

5. Discussion

Author details

References

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