In Vitro Blood Compatibility of Novel Hydrophilic Chitosan Films for Vessel Regeneration and Repair ( Advances in Biomaterials Science and Biomedical Applications )

Publication series ： Advances in Biomaterials Science and Biomedical Applications

Author： Antonello A. Romani Luigi Ippolito Federica Riccardi Silvia Pipitone  

Publisher： IntechOpen‎

Publication year： 2013

E-ISBN: INT5642052706

P-ISBN(Paperback): 9789535110514

P-ISBN(Hardback):  9789535110514

Subject： R3 Basic Medical

Keyword： 基础医学

Language： ENG

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In Vitro Blood Compatibility of Novel Hydrophilic Chitosan Films for Vessel Regeneration and Repair

Chapter

Chapter 6 In Vitro Blood Compatibility of Novel Hydrophilic Chitosan Films for Vessel Regeneration and Repair

1. Introduction

2. Methods

2.1. Production of sugar-modified chitosan films

2.2. Wettability

2.3. Atomic force microscopy

2.4. Procurement and processing of blood perfusates

2.5. Cell proliferation

2.6. Cell morphology

2.7. Cytotoxicity test

2.8. Haemolysis assay

2.9. Coagulation assays

2.10. Erythrocytes adhesion assay

2.11. Platelet adhesion assay

3. Platelet immunofluorescence

4. Platelet morphology

4.1. Platelet aggregation

4.2. Complement activation assay

4.3. Statistical analysis

5. Results

5.1. Physico-chemical characterisation

5.2. Wettability

5.3. Roughness (AFM measurements)

5.4. Adhesion and proliferation assay of endothelial cells

5.5. In vitro cytotoxicity

5.6. Haemolysis assay

5.7. Blood coagulation assay

5.8. Complement activation assay

5.9. Erythrocytes and platelets adhesion assay

5.10. Platelet aggregation

5.11. Platelet activation assay

6. Discussion

7. Conclusion

Acknowledgements

Author details

References

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