Chapter 5 Connexin36 is a Negative Regulator of Differentiation in Human Neuroblastoma

1. Introduction

2. Experimental procedures

2.1. Cell culture

2.2. Generation of stable cell lines

2.2.1. Overexpression

2.2.2. Knockdown

2.3. Differentiation assay

2.4. Real-time PCR

2.5. Western blot analysis

2.6. MTT proliferation assay

2.7. Statistical analysis

3. Results

3.1. Retinoic acid induces differentiation and upregulates Cx36 expression of SH-SY5Y neuroblastoma cells

3.2. Cx36 suppresses neuritogenesis

3.3. Cx36 suppresses differentiation marker expression

3.4. Cx36 promotes cell clumping

3.5. Cx36 increases SH-SY5Y cell proliferation

4. Discussion

4.1. Retinoic acid induces upregulation of Cx36

4.2. Cx36 negatively regulates SH-SY5Y cell differentiation

4.2.1. Cx36 promotes clumping of SH-SY5Y cells

4.2.2. Cx36 increases cell proliferation

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References