In vivo Administration of Granulocyte Colony-Stimulating Factor Increases the Surface Expression of Sialyl-Lewisx on Neutrophils in Healthy Volunteers

Publisher: Karger

E-ISSN: 1421-9662|100|4|187-190

ISSN: 0001-5792

Source: Acta Haematologica, Vol.100, Iss.4, 1999-02, pp. : 187-190

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Abstract

We examined the in vivo effect of granulocyte colony-stimulating factor (G-CSF) on the surface expression of putative counterligands for endothelial selectins on neutrophils in healthy volunteers. G-CSF (50 µg/m2/day) was administered subcutaneously to 5 healthy volunteers for 4 days. The expression of surface antigens on neutrophils was determined by flow cytometry and monoclonal antibodies. G-CSF administration increased the number of leukocytes, mainly of neutrophils, which was associated with an increase in the expression of the high-affinity Fc receptor for IgG (FcRI, CD64) and CD14 on neutrophils. G-CSF administration decreased the surface expression of L-selectin on neutrophils, whereas it increased the expression of sialyl-Lewisx but not Lewisx on neutrophils. These findings suggest that G-CSF participates in the neutrophil-endothelial cell interactions in vivo by modulating the expression of adhesion molecules and ligands for endothelial selectins on neutrophils.