ZEBRAFISH: TOPICS IN REPRODUCTION, TOXICOLOGY AND DEVELOPMENT

ZEBRAFISH: TOPICS IN REPRODUCTION, TOXICOLOGY AND DEVELOPMENT

Library of Congress Cataloging-in-Publication Data

CONTENTS

PREFACE

PART 1: REPRODUCTION

Chapter 1: AGE DETERMINATION OF GONAD MATURATION AND PUBERTY ONSET IN THE TRANSPARENT CASPER ZEBRAFISH JUVENILE

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

ACKNOWLEDGEMENTS

REFERENCES

Chapter 2: OVARIAN FOLLICLE DYNAMICS ASSESSED IN VIVO BY INTRAPERITONEAL (I.P.) TRYPAN BLUE UPTAKE DURING VITELLOGENIN ENDOCYTOSIS IN ADULT FEMALE ZEBRAFISH (DANIO RERIO)

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

Chapter 3: THE PROTEIN PHOSPHATASE INHIBITOR, OKADAIC ACID, ELICITS SEVERAL COMPONENTS OF ZEBRAFISH (DANIO RERIO) OOCYTE MATURATION IN VITRO

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

Chapter 4: GET IT TOGETHER: HOW RNA-BINDING PROTEINS ASSEMBLE AND REGULATE GERM PLASM IN THE OOCYTE AND EMBRYO

ABSTRACT

INTRODUCTION

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

Chapter 5: ZEBRAFISH AS A MODEL FOR REPRODUCTIVE BIOLOGY AND ENVIRONMENTAL SCREENING

ABSTRACT

INTRODUCTION

ESTABLISHMENT OF OVARIAN FLUORESCENT TRANSPARENT STRAIN

IN VITRO OOCYTE MATURATION ASSAY FOR NONGENOMIC STEROID ACTIONS

IN VIVO SCREENING FOR CHEMICAL EFFECTS ON FISH REPRODUCTION - AN ASSAY FOR NONGENOMIC STEROID ACTIONS

SEX-REVERSAL IN ADULT FEMALE -PRESENCE OF UNDIFFERENTIATED STEM CELLS FOR THE GERMLINE IN ADULT ZEBRAFISH

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

Chapter 6: FECUNDITY AND SPAWNING PERIODICITY IN WILD-TYPE ZEBRAFISH MATED PAIRS: A LONG-TERM, LONGITUDINAL STUDY

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

PART 2: DEVELOPMENT

Chapter 7: LOCALIZATION OF THE SODIUM-POTASSIUM-CHLORIDE COTRANSPORTER (SLC12A2) DURING ZEBRAFISH EMBRYOGENESIS AND MYOGENESIS AND A SCREEN FOR ADDITIONAL ANTIBODIES TO STUDY ZEBRAFISH MYOGENESIS

ABSTRACT

INTRODUCTION

METHODS

RESULTS

DISCUSSION

CONCLUSION

ACKNOWLEDGEMENTS

REFERENCES

Chapter 8: THE ZEBRAFISH DEAD ELVIS (DEL) MUTANT ENCODES TITINA

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

ACKNOWLEDGEMENTS

REFERENCES

Chapter 9: RENAL SYSTEM DEVELOPMENT IN THE ZEBRAFISH: A BASIC NEPHROGENESIS MODEL

ABSTRACT

ABBREVIATIONS

INTRODUCTION

NEPHRON STRUCTURE AND ORGANIZATIONAL CONSERVATION AMONG VERTEBRATES

INSIGHTS INTO NEPHROGENESIS USING THE ZEBRAFISH

FURTHER DELINEATION OF RENAL DEVELOPMENTAL PATHWAYS WITH ESTABLISHED AND EMERGING MOLECULAR TOOLS IN ZEBRAFISH

CONCLUSION

REFERENCES

Chapter 10: THE USE OF WHOLE MOUNT IN SITUHYBRIDIZATION SCREENING TO UNDERSTAND THE DEVELOPMENTAL TOXICOLOGY OF ENVIRONMENTAL POLLUTANTS IN ZEBRAFISH EMBRYOS

ABSTRACT

ABBREVIATIONS

INTRODUCTION

SUGGESTED PROCEDURE FOR TOXICANTS TEST IN ADULT FISH

SUGGESTED PROCEDURE FOR TOXICANTS SCREENINGS IN ZEBRAFISH EMBRYOS IN 96-WELL PLATE

CONCLUSION AND PROSPECTIVE

REFERENCES

Chapter 11: USING ZEBRAFISH TO DEFINE MECHANISMS OF LEAD (Pb) DEVELOPMENTAL NEUROTOXICITY

ABSTRACT

INTRODUCTION

PB MECHANISMS OF NEUROTOXICTY

PB NEUORTOXICITY IN RODENT MODELS

PB AND DEVELOPMENTAL ORIGINS OF ADULT DISEASE (DOAD)

ZEBRAFISH: A COMPLEMENTARY VERTEBRATE MODEL ORGANISM

PB NEUROTOXICITY IN ZEBRAFISH

CONCLUSION

REFERENCES

Chapter 12: THE EMBRYONIC ZEBRAFISH AS A MODEL SYSTEM TO STUDY THE EFFECTS OF ENVIRONMENTAL TOXICANTS ON BEHAVIOR

ABSTRACT

INTRODUCTION

THE DEVELOPING ZEBRAFISH AS A MODEL SYSTEM FOR ENVIRONMENTAL TOXICOLOGY

EMBRYONIC BEHAVIOR ASSESSMENTS

CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

Chapter 13: ACUTE TOXICITY AND STUDY OF “BIOMARKER OF EFFECTS” IN ZEBRAFISH EMBRYOS AND LARVAE EXPOSED TO SELECTED PESTICIDES: A STEP TOWARDS REFINED RISK ASSESSMENT OF CHEMICAL AGENTS

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGMENTS

REFERENCES

INDEX