Front Cover

Advances in Cellular Neurobiology

Copyright Page

Table of Contents

LIST OF CONTRIBUTORS

PREFACE

SECTION 1: CELL DIFFERENTIATION AND INTERACTION

CHAPTER 1. SPECIALIZATIONS OF NONNEURONAL CELL MEMBRANES IN THE VERTEBRATE NERVOUS SYSTEM

I. Introduction

II. Gap Junctions

III. Tight Junctions

IV. Assemblies

References

CHAPTER 2. EFFECTS OF NEUROHORMONES ON GLIAL CELLS

I. Introduction

II. Models for Glial Cells

III. Receptors for Putative Neurohormones

IV. Events Secondary to Receptor Activation

V. Conclusions

References

CHAPTER 3. RETROGRADE AXONAL TRANSPORT

I. Introduction

II. Mechanism of Retrograde Axonal Transport

III. Retrograde Transport of Materials Endogenous to the Neuron

IV. Retrograde Transport of Materials Exogenous to the Neuron

V. Functions of Retrograde Transport of Exogenous Materials

VI. Conclusion

References

CHAPTER 4. BIOCHEMICAL CHARACTERISTICS OF INDIVIDUAL NEURONS

I. Introduction

II. Single-Cell Samples

III. Cell Structure

IV. Biochemical Components

V. Conclusion

References

SECTION 2. AGING AND PATHOLOGY

CHAPTER 5. CEREBELLAR GRANULE CELLS IN NORMAL AND NEUROLOGICAL MUTANTS OF MICE

I. Introduction

II. Possible Effect of Purkinje Cells on Proliferation of Granule Cells

III. Effect of Glial Cells on Granule Cell Migration

IV. Survival of Granule Cells in Mutant Mice (Staggerer and Weaver)

V. Parallel Fiber–Purkinje Cell Synapses

VI. Granule Cell Transmitter

VII. Comments on the Use of Neurological Mutants as Model Systems

References

CHAPTER 6. CELL GENERATION AND AGING OF NONTRANSFORMED GLIAL CELLS FROM ADULT HUMANS

I. Cell Generation and Aging

II. Origin of Adult Human Glia-Like Cell Lines

III. Theories about Cellular Aging

IV. Basic Characteristics of Adult Human Glia-Like Cells in Vitro

V. Relation between Cell Generation and Aging in Human Glia-Like Cells

VI. Miniclone Analysis of Glia-Like Cells

VII. Conclusions

References

CHAPTER 7. AGE-RELATED CHANGES IN NEURONAL AND GLIAL ENZYME ACTIVITIES

I. General Introduction

II. Neuron-Specific Enzymes

III. Glia-Specific Enzymes

IV. Enzymes Associated with Specific Cellular Processes

References

CHAPTER 8. GLIAL FIBRILLARY ACIDIC (GFA) PROTEIN IN NORMAL NEURAL CELLS AND IN PATHOLOGICAL CONDITIONS

I. Introduction

II. Biochemical Properties of GFA Protein

III. Preparation of Antisera

IV. Immunohistochemical Localization of GFA Protein in Adult CNS

V. Gliogenesis

VI. Astroglial Response to Injury

VII. Astroglial Marker in Vitro

VIII. Diagnosis of Brain Tumors

References

SECTION 3. METHODOLOGIES

CHAPTER 9. IN VITRO BEHAVIOR OF ISOLATED OLIGODENDROCYTES

I. Introduction

II. Isolation of Oligodendrocytes

III. Oligodendrocyte Subpopulations

IV. Culture of Oligodendrocytes

V. Conclusions

References

CHAPTER 10. BIOCHEMICAL MAPPING OF SPECIFIC NEURONAL PATHWAYS

I. Introduction

II. Techniques for Biochemical Classification of Neurons

III. Identification of Interconnections between Neurons Using Different Transmitters

References

CHAPTER 11. SEPARATION OF NEURONAL AND GLIAL CELLS AND SUBCELLULAR CONSTITUENTS

I. Introduction

II. Methods of Cell Isolation

III. Subcellular CNS Fractions

IV. New Isolation Techniques

V. The Use of Cell Fractions

References

CHAPTER 12. SEPARATION OF NEURONS AND GLIAL CELLS BY AFFINITY METHODS

I. Introduction

II. Techniques of Separation by Affinity Systems

III. Ligands for Neural Cell Surfaces

IV. Concluding Remarks

References

Subject Index