Description
G Protein Pathways is the first of three volumes examining the nature of heterotrimeric G proteins. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.
Chapter
Section I: G Protein-Coupled Receptors
A. Theoretical Evaluation of Receptor Function
Chapter 1. Considerations in the Evaluation of Inverse Agonism and Protean Agonism at GProtein-Coupled Receptors
Chapter 2. Theoretical Implications of Receptor Coupling to Multiple G Proteins Based on Analysis of Three-State Model
B. Design and Use of Receptor Ligands
Chapter 3. Use of Retinal Analogues for the Study of Visual Pigment Function
Chapter 4. Design and Synthesis of Peptide Antagonists and Inverse Agonists for G Protein-Coupled Receptors
Chapter 5. Design of Peptide Agonists
Chapter 6. Design of Nonpeptides from Peptide Ligands for Peptide Receptors
Chapter 7. Strategies for Mapping the Binding Site of the Serotonin 5-HT2A Receptor
C. Structural Characterization of Receptor Proteins
Chapter 8. Use of the Substituted Cysteine Accessibility Method to Study the Structure and Function of G Protein-Coupled Receptors
Chapter 9. Mass Spectrometric Analysis of GProtein-Coupled Receptors
Chapter 10. Probing the Higher Order Sructure of G Protein- Coupled Receptors Using Tethered Cleavage Methods
Chapter 11. Use of Fluorescence Spectroscopy to Study Conformational Changes in the β2-Adrenoceptor
Chapter 12. Crystallization of Membrane Proteins in Cubo
Chapter 13. N-Linked Carbohydrates on G Protein-Coupled Receptors: Mapping Sites of Attachment and Determining Functional Roles
Chapter 14. Magic Angle Spinning Nuclear Magnetic Resonance of Isotopically Labeled Rhodopsin
Chapter 15. Use of Nuclear Magnetic Resonance to Study the Three-Dimensional Structure of Rhodopsin
D. Design and Use of Engineered Receptor Proteins
Chapter 16. Tools for Dissecting Signaling Pathways in Vivo: Receptors Activated Solely by Synthetic Ligands
Chapter 17. Analysis of Structure–Function from Expression of G Protein-Coupled Receptor Fragments
Chapter 18. Construction and Analysis of Function of G Protein-Coupled Receptor–G Protein Fusion Proteins
Chapter 19. Synthetic Gene Technology: Applications to Ancestral Gene Reconstruction and Structure Function Studies of Receptors
Chapter 20. Considerations in the Design and Use of Chimeric G Protein-Coupled Receptors
E. Molecular Modeling Studies of Receptor Structure and Function
Chapter 21. Strategies for Modeling the Interactions of Transmembrane Helices of G Protein-Coupled Receptors by Geometric Complementarity Using the GRAMM Computer Algorithm
Chapter 22. Three-Dimensional Representations of G Protein- Coupled Receptor Structures and Mechanisms
F. Analysis of Receptor Protein Coupling
Chapter 23. Reconstitution of G Protein-Coupled Receptors with Recombinant GProteinαand βγ Subunits
Chapter 24. Cell-Free Membrane Desensitization Assay for G Protein-Coupled Receptors
Chapter 25. Methods to Determine the Constitutive Activity of Histamine H2 Receptors
Chapter 26. Expression of G Protein-Coupled Receptors and G Proteins in Sf9 Cells: Analysis of Coupling by Radioligand Binding
Chapter 27. G Protein-Coupled Receptors and Proliferative Signaling
G. Characterization of Receptor Heterogeneity
Chapter 28. Genetic Analysis of G Protein-Coupled Receptor Genes
Chapter 29. IdentiIfication of Adrenergic Receptor Polymorphisms
Chapter 30. Strategies and Requirements for the Detection of RNA Editing in G Protein Coupled-Receptor RNA
H. The Study of Receptor Trafficking
Chapter 31. Fluorescence MicroscopyTechniques for the Study of G Protein-Coupled Receptor Trafficking
Chapter 32. Measurement of Receptor Desensitization and Internalization in Intact Cells
Chapter 33. Morphological and Biochemical Strategies for Monitoring Trafficking of Epitope-Tagged G Protein-Coupled Receptors in Agonist- Naive and Agonist-Occupied States
Section II: Regulators of GPCR Function
A. G Protein-Coupled Receptor Kinases (GRKs)
Chapter 34. Characterization of G Protein-Coupled Receptor Kinases
Chapter 35. Regulation of G Protein-Coupled Receptor Kinase 2
Chapter 36. Rhodopsin and Its Kinase
B. Arrestins and Novel Proteins
Chapter 37. Characterization of Arrestin Expression and Function
Chapter 38. Identification of Novel G Protein-Coupled Receptor-Interacting Proteins
G Protein Pathways, Part A: Receptors
：Receptors
( Volume 343 )
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