Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|62|12|1209-1211
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.62, Iss.12, 2006-12, pp. : 1209-1211
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Abstract
6‐Aminohexanoate‐cyclic‐dimer hydrolase (EI) from Arthrobacter sp. KI72 was expressed in Escherichia coli and purified by anion‐exchange chromatography. EI was crystallized by the sitting‐drop vapour‐diffusion method with sodium citrate as precipitant in imidazole buffer pH 8.0. The crystal is hexagonal, with unit‐cell parameters a = b = 130.75, c = 58.23 Å. Diffraction data were collected from native and mercury(II) dichloride‐derivative crystals to resolutions of 1.90 and 2.06 Å, respectively.
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