Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|64|11|1052-1055
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.64, Iss.11, 2008-11, pp. : 1052-1055
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Abstract
AcsD, a type A siderophore synthetase with a molecular weight of 71 140 Da from Pectobacterium chrysanthemi, has been expressed, purified and crystallized at 293 K. The protein crystallized in the primitive orthorhombic space group P212121, with unit‐cell parameters a = 80.3, b = 95.7, c = 161.1 Å, α = β = γ = 90°. Systematic absences were consistent with space group P212121. A complete data set has been collected to 2.25 Å resolution on BM14 at the ESRF. Consideration of the likely solvent content suggested that the asymmetric unit contained two molecules. Gel‐filtration experiments indicated that the protein was a dimer, although self‐rotation analyses did not detect a convincing twofold symmetry axis in the asymmetric unit. The protein has no convincing sequence match to any known structure and thus solution is likely to require experimental phasing.
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