Author: Li Xi Luo He-Sheng Paul Shelley C. Tang Tao Yuan Guang-Jin
Publisher: MDPI
E-ISSN: 1422-0067|7|2|59-70
ISSN: 1422-0067
Source: International Journal of Molecular Sciences, Vol.7, Iss.2, 2006-02, pp. : 59-70
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
The regulation of glucose and sodium butyrate transporters(glucose transporter1-5 and Monocarboxylate transporter 1) and their relationship with cell apoptosis induced by sodium butyrate in colonic caner cell line HT-29 were studied. Cell apoptosis was detected by flow cytometric assay. The expression of MCT1 and GLUT1-5 mRNA were detected by RT-PCR and the uptake of glucose was detected using 2-deoxy-[3H]glucose. The expression of bax and bcl-x/l were detected by westernblot assay. We found that sodium butyrate induced apoptosis in HT-29 cell line. The expression of GLUT1 mRNA, bcl-x/l, as well the uptake of glucose was inhibited by sodium butyrate. The expression of MCT1 and GLUT2, GLUT3, GLUT5 was not regulated by sodium butyrate. However, the concentration of glucose had positive correlation with the expression of bcl-x/l protein and negative correlation with the apoptosis induced by sodium butyrate. All the results suggested thatdownregulation of the expression of GLUT1 was associated with the apoptosis induced by sodium butyrate inHT-29 cell line.
Related content
By Shin Eun Ju Sung Mi Jeong Park Jae Ho Yang Hye Jeong Kim Myung Sunny Hur Haeng Jeon Hwang Jin-Taek
International Journal of Molecular Sciences, Vol. 16, Iss. 4, 2015-04 ,pp. :
By Ren Bao-Jun Zhou Zhi-Wei Zhu Da-Jian Ju Yong-Le Wu Jin-Hao Ouyang Man-Zhao Chen Xiao-Wu Zhou Shu-Feng
International Journal of Molecular Sciences, Vol. 17, Iss. 1, 2015-12 ,pp. :
By Lay Ma Ma Karsani Saiful Anuar Abd Malek Sri Nurestri
International Journal of Molecular Sciences, Vol. 15, Iss. 1, 2014-01 ,pp. :
By Zhang Li Jiang Fen Chen Yuanhan Luo Jialun Liu Shuangxin Zhang Bin Ye Zhiming Wang Wenjian Liang Xinling Shi Wei
International Journal of Molecular Sciences, Vol. 14, Iss. 12, 2013-12 ,pp. :