Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris

Author： Shi Bihong   Zeng Liqing   Song Haolei   Shi Qiaoqin   Wu Songgang  

Publisher： MDPI

E-ISSN： 1422-0067|11|6|2373-2382

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.11, Iss.6, 2010-06, pp. : 2373-2382

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Abstract

A lipase gene (atl) was cloned from Aspergillus tamarii FS132 for the first time. The gene was found to have an open reading frame of 1024 base pairs (bp), and the coding region of the gene contained two introns (51 bp and 52 bp). Multi-alignment analysis of the deduced amino acid sequence indicated high homology between the enzyme and mono-and diacylglycerol lipases from fungi Aspergillus. The recombinant lipase was expressed in Pichia pastoris GS115 cells. The recombinant lipase was found to have a molecular mass of 36.7 kDa, and it exhibited lipase activity of 20 U/mL in culture supernatant when tributyrin was used as the substrate.

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