Author： Zhang Zhi-Guo Sun Xin Zhang Qing-Zhu Yang Hua
Publisher： MDPI
E-ISSN： 1422-0067|14|1|1932-1939
ISSN： 1422-0067
Source： International Journal of Molecular Sciences, Vol.14, Iss.1, 2013-01, pp. : 1932-1939
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Previous experiments showed that ultra-low-molecular-weight heparin (ULMWH) reduced the infarct and neurologic deficit in rats followed by transient cerebral ischemia, but the mechanisms of its neuroprotective effect are unclear. This study reported the effect of ULMWH on energy metabolism, inflammatory reaction and neuronal apoptosis. Male Wistar rats were subjected to middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion for 24 h. ULMWH (0.5, 1 mg/kg, i.v.) was administered after the MCAO and reperfusion. 24 h after the reperfusion, Spectrophotometric assay was used to determine the activity of ATPase and the content of lactic acid in the brain. The ICAM-1 and Caspase-3 genes were investigated by RT-PCR. Furthermore, the apoptotic percentage of cells in hippocampus was quantified by flow cytometry. Compared with the model group, ULMWH significantly decreased lactic acid content and increased ATPase activity in ischemic brain. At the same time, ULMWH inhibited the neural apoptosis and decreased the expressions of ICAM-1 and Caspase-3 mRNA in hippocampus. These findings suggest that ULMWH exhibits a neuroprotective effect against cerebral ischemia/reperfusion injury, partly through improving energy metabolism, inhibiting apoptosis and attenuating inflammatory reaction.
Related content
Access to resources
Recommend
By Zhang Weiqiao Miao Yifeng Zhou Sanquan Wang Baofeng Luo Qizhong Qiu Yongming
International Journal of Molecular Sciences, Vol. 11, Iss. 11, 2010-11 ,pp. :
By Hao Lingyun Wei Xuewen Guo Peng Zhang Guangyi Qi Suhua
International Journal of Molecular Sciences, Vol. 17, Iss. 7, 2016-07 ,pp. :
By Pei Haitao Cao Dongming Guo Zhuangli Liu Guofang Guo Yunliang Lu Chenglong
International Journal of Molecular Sciences, Vol. 14, Iss. 12, 2013-11 ,pp. :