Author： Tian Shan Bai Yulong Yang Lin Wang Xinggang Wu Yi Jia Jie Zhu Yulian Cheng Yong Zhang Pengyue Wu Junfa Wang Nianhong Xia Guang Liao Hua Zhang Yuling Shen Xiafeng Yu Huixian Hu Yongshan
Publisher： MDPI
E-ISSN： 1422-0067|14|1|1412-1427
ISSN： 1422-0067
Source： International Journal of Molecular Sciences, Vol.14, Iss.1, 2013-01, pp. : 1412-1427
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Abstract
As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
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