Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

Author： He Jinzhe Zhang Anqiang Ru Qiaomei Dong Dandan Sun Peilong

Publisher： MDPI

E-ISSN： 1422-0067|15|1|787-797

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.15, Iss.1, 2014-01, pp. : 787-797

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Abstract

An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 10⁴ Da, which was named ABP, and its main components were d-glucose and d-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D ¹H and ¹³C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-d-Glcp as the main backbone with (1→4)-linked α-d-Manp units. The structure of the mainly repeating units of ABP was established as:→6)-β-D-Glucp-(1→4)-α-D-Manp(1→6)-β-D-Glucp-(1→6)-β-D-Glucp-(1→