Author： Troppan Katharina Wenzl Kerstin Pichler Martin Pursche Beata Schwarzenbacher Daniela Feichtinger Julia Thallinger Gerhard G. Beham-Schmid Christine Neumeister Peter Deutsch Alexander
Publisher： MDPI
E-ISSN： 1422-0067|16|8|18077-18095
ISSN： 1422-0067
Source： International Journal of Molecular Sciences, Vol.16, Iss.8, 2015-08, pp. : 18077-18095
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Micro-RNAs (miRNAs) are short non-coding single-stranded RNA molecules regulating gene expression at the post-transcriptional level. miRNAs are involved in cell development, differentiation, apoptosis, and proliferation. miRNAs can either function as tumor suppressor genes or oncogenes in various important pathways. The expression of specific miRNAs has been identified to correlate with tumor prognosis. For miRNA expression analysis real-time PCR on 81 samples was performed, including 63 diffuse large B-cell lymphoma (DLBCL, 15 of germinal center B-cell like subtype, 17 non germinal center B-cell, 23 transformed, and eight unclassified) and 18 controls, including nine peripheral B-cells, 5 germinal-center B-cells, four lymphadenitis samples, and 4 lymphoma cell lines (RI-1, SUDHL4, Karpas, U2932). Expression levels of a panel of 11 miRNAs that have been previously involved in other types of cancer (miR-15b_2, miR-16_1*, miR-16_2, miR-16_2*, miR-27a, miR-27a*, miR-98-1, miR-103a, miR-185, miR-199a, and miR-497) were measured and correlated with clinical data. Furthermore, cell lines, lacking miR-199a and miR-497 expression, were electroporated with the two respective miRNAs and treated with standard immunochemotherapy routinely used in patients with DLBCL, followed by functional analyses including cell count and apoptosis assays. Seven miRNAs (miR-16_1*, miR-16_2*, miR-27a, miR-103, miR-185, miR-199, and miR-497) were statistically significantly up-regulated in DLBCL compared to normal germinal cells. However, high expression of miR-497 or miR-199a was associated with better overall survival (
Related content
Access to resources
Recommend
By Lv Pin Zhou Mingxia He Jing Meng Weiwei Ma Xuehan Dong Shuling Meng Xianchun Zhao Xue Wang Xi He Fucheng
International Journal of Molecular Sciences, Vol. 15, Iss. 4, 2014-04 ,pp. :
Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma
By Fedorko Michal Stanik Michal Iliev Robert Redova-Lojova Martina Machackova Tana Svoboda Marek Pacik Dalibor Dolezel Jan Slaby Ondrej
International Journal of Molecular Sciences, Vol. 16, Iss. 10, 2015-09 ,pp. :