Author: Zhou Hao Chen Shun Zhou Qin Wei Yunan Wang Mingshu Jia Renyong Zhu Dekang Liu Mafeng Liu Fei Yang Qiao Wu Ying Sun Kunfeng Chen Xiaoyue Cheng Anchun
Publisher: MDPI
E-ISSN: 1999-4915|8|7|195-195
ISSN: 1999-4915
Source: Viruses, Vol.8, Iss.7, 2016-07, pp. : 195-195
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Abstract
Interferons are a group of antiviral cytokines acting as the first line of defense in the antiviral immunity. Here, we describe the antiviral activity of goose type I interferon (IFNα) and type II interferon (IFNγ) against duck plague virus (DPV). Recombinant goose IFNα and IFNγ proteins of approximately 20 kDa and 18 kDa, respectively, were expressed. Following DPV-enhanced green fluorescent protein (EGFP) infection of duck embryo fibroblast cells (DEFs) with IFNα and IFNγ pre-treatment, the number of viral gene copies decreased more than 100-fold, with viral titers dropping approximately 100-fold. Compared to the control, DPV-EGFP cell positivity was decreased by goose IFNα and IFNγ at 36 hpi (3.89%; 0.79%) and 48 hpi (17.05%; 5.58%). In accordance with interferon-stimulated genes being the “workhorse” of IFN activity, the expression of duck myxovirus resistance (Mx) and oligoadenylate synthetases-like (OASL) was significantly upregulated (
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