Author: Zhang Yue Xiao Wei Kong Hui Cheng Jinjun Yan Xin Zhang Meiling Wang Qingguo Qu Huihua Zhao Yan
Publisher: MDPI
E-ISSN: 1420-3049|23|2|338-338
ISSN: 1420-3049
Source: Molecules, Vol.23, Iss.2, 2018-02, pp. : 338-338
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Abstract
A quantitative lateral-flow immunoassay using gold nanoparticles (AuNPs) conjugated with a monoclonal antibody (MAb) against saikosaponin d (SSd) was developed for the analysis of SSd. The AuNPs were prepared in our laboratory. The AuNPs were polyhedral, with an average diameter of approximately 18 nm. We used the conjugation between AuNPs and MAbs against SSd to prepare immunochromatographic strips (ICSs). For the quantitative experiment, the strips with the test results were scanned using a membrane strip reader, and a detection curve (regression equation,
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