B7H3 promotes cell migration and invasion through the Jak2/Stat3/MMP9 signaling pathway in colorectal cancer

Author： Liu Fen Zhang Ting Zou Shitao Jiang Bo Hua Dong

E-ISSN： 1791-3004|12|4|5455-5460

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.12, Iss.4, 2015-10, pp. : 5455-5460

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Abstract

B7H3, a newly identified costimulatory molecule, has been reported to be highly expressed in a number of types of cancer and is associated with a poor prognosis. Transwell experiments and a wound-healing assay were used to detect the role of overexpressed B7H3 on cell migration and invasion in colorectal cancer (CRC) cells. The expression level of matrix metallopeptidase 9 (MMP9) was further investigated by zymography experiments and western blot analysis, and involvement of the Janus kinase 2 (Jak2) signal transducer and activator of transcription 3 (STAT3) signaling pathway was determined using AG490, a Jak2 selective inhibitor. Data showed that overexpression of B7H3 promoted cell migration and invasion in CRC. Further investigation certified that enhanced expression of B7H3 elevated MMP9 through upregulation of the Jak2Stat3 signaling pathway. Due to its promigratory and proinvasive function, B7H3 may serve as a therapeutic target in the treatment of CRC.