Description
Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which have not been published in full form elsewhere in the literature. The presentations in this volume are comprehensive to the extent that they may serve not only as a practical introduction to experimental procedures but they also provide some evaluation of the limitations, potentialities, and current applications of the methods. Only those theoretical considerations needed for proper use of the method are included.
The book may have particular usefulness for those working with intact cells, and the first chapters deal with culturing and experimental manipulation of a variety of cell types. There are numerous descriptions of techniques designed for working with single cells and of procedures for studying cellular activities in relation to the cell life cycle. The chapters on autoradiography include detailed treatment of qualitative and quantitative aspects of the method as well as simple procedures for using liquid and dry emulsions.
Chapter
II. Culture of Impure Myxomycetes
III. Isolation and Pure Culture
IV. Axenic Culture of Physarum polycephalum on Soluble Media
V. Sporulation (Fruiting) of Physarum polycephalum in Pure Culture
VI. Sclerotia and Spherule Formation
VII. Changes Observed after Prolonged Growth in Pure Culture
Chapter 3. Mitotic Synchrony in the Plasmodia of Physarum polycephalum and Mitotic Synchronization by Coalescence of Microplasmodia
II. Life History of Physarum polycephalum
III. The Experimental Material
Chapter 4. Induction of Synchronous Encystment (Differentiation) in Acanthamoeba sp.
II. The Encystment Process: A Description
III. Induction of Synchronous Encystment: The Method
IV. Optimal Conditions for Synchronous Encystment
V. Evaluation of the Method
Chapter 5. Experimental Procedures and Cultural Methods for Euplotes eurystomus and Amoeba proteus
Chapter 6. Nuclear Transplantation in Ameba
Chapter 7. Experimental Techniques with Ciliates
II. Experimental Possibilities with Stentor
III. Techniques with Paramecium
Chapter 8. Methods for Using Tetrahymena in Studies of the Normal Cell Cycle
III. Manipulation of Cells
Chapter 9. Continuous Synchronous Cultures of Protozoa
II. Light-Induced Synchrony in Euglena gracilis
III. Temperature-Induced Synchrony in Astasia longa
Chapter 10. Handling and Culturing of Chlorella
II. The Organisms and Their growth
III. The Culture of Chlorella
Chapter 11. Culturing and Experimental Manipulation of Acetabularia
I. Introduction to the Organism
V. Potentialities and Limitations
Chapter 12. Handling of Root Tips
II. Germination of Seeds and Growing of Roots
III. Fixation of Root Tips
V. Making Slides Permanent
VI. Autoradiographic Methods
Chapter 13. Grasshopper Neurohlast Techniques
VIII. Separated Cell Techniques
IX. Fixed Cell Techniques
Chapter 14. Measurement of Material Uptake by Cells: Pinocytosis
II. Demonstration of Pinocytosis
III. Inducers of Pinocytosis
IV. Quantitative Estimation of Pinocytic Uptake
V. Significance of the Values Obtained and Errors Likely to be Encountered in the Different Methods
Chapter 15. Quantitative Autoradiography
II. Theoretical Considerations
III. Practical Considerations and Techniques
IV. The Problem of "Intermediates"
Chapter 16. High-Resolution Autoradiography
III. Discussion of the Methods
Chapter 17. Autoradiography with Liquid Emulsion
Chapter 18. Autoradiography of Water-Soluble Materials
III. Experimental Example
Chapter 19. Preparation of Mammalian Metaphase Chromosomes for Autoradiography
III. Examples and Variation of Technique
Chapter 20. Methods for Measuring the Length of the Mitotic Cycle and the Timing of DNA Synthesis for Mammalian Cells in Culture
II. Measurements of Intermitotic Times by Time-Lapse Cinemicrography
III. Measurements of Growth Rates of Cultures
IV. Measurement of Parts of Interphase
Chapter 21. Micrurgy of Tissue Culture Cells
Chapter 22. Microextraction and Microelectrophoresis for Determination and Analysis of Nucleic Acids in Isolated Cellular Units