Description
Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achievements in areas such as electrochemical immunosensors, nanoprobe-based immunoassay, chemiluminescence immunoassay, electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging for immunoassay, signal amplification for immunoassay, and so on.
In recent years, immunosensing and immunoassay methods have attracted considerable interest due to their applications in different fields, particularly clinical diagnosis. Although a large number of academic papers in immunosensing and immunoassay have been published in different journals recently, it is still a difficult and time-consuming task for researchers, especially those new to the area, to understand the principles, methods, and research progress of immunosensing.
Based on the research experience of the authors and their research groups, this book offers readers with new research ideas to develop immunosensing methodology. As a monograph, it offers deeper and more complete coverage than review papers, which only report certain aspects of progress. Grounded in the research experience of Professor Ju’s research group, the book focuses on immunosensing for detection of protein biomarkers, summarizing understanding, research, and practice on immunosensing methodology in detection of protein biomarkers.
Chapter
1.2.1 Optical immunosensors
1.2.2 Electrochemical immunosensors
1.2.3 Mass-sensitive immunosensors
1.3 Immobilization method of immunoreagents
1.3.1 Physical adsorption
1.3.4 Oriented immobilization
1.4 Signal labels used in immunoassay
1.4.2 Noble-metal nanoparticles
1.4.3 Electrochemically or optically active agents
2 Signal amplification for immunosensing
2.1 Multilabeling for signal amplification
2.2 Nanoparticles as signal labels for signal amplification
2.3 Enzyme mimics for signal amplification
2.4 PCR for signal-amplified immunosensing
2.5 Rolling circle amplification for amplified immunoassay
2.6 HCR for amplified immunoassay
3 Electrochemical immunosensing
3.2 Direct immunoreaction-based immunosensors
3.3 Competitive immunoreaction-based immunosensors
3.4 Sandwich-type immunosensors
3.5 Direct electrochemistry of enzyme for immunosensing
4 Functional nanoprobes for immunosensing
4.1 Nanoprobe as signal labels
4.2 Enzyme-functionalized nanoprobes
4.3 Noble-metal nanoparticle-functionalized nanoprobes
4.4 QD functionalized nanoprobes
4.5 Other signal label functionalized nanoprobes
5 Chemiluminescent immunoassay
5.4 Capillary electrophoresis for CL immunoassay
6 Electrochemiluminescent immunosensing
6.1 Electrochemiluminescence and electrochemiluminescent emitters
6.1.1 ECL emission mechanism of nanomaterials
6.1.2 Miscellaneous ECL nanoemitters
6.2 Label-free electrochemiluminescent immunoassay
6.2.1 Carbon nanomaterials enhanced label-free ECL immunoassay
6.2.2 Metallic nanoparticles enhanced label-free ECL immunoassay
6.2.3 Other nanomaterials enhanced label-free ECL immunoassay
6.3 “Signal-off” electrochemiluminescent immunoassay
6.3.1 Enzyme label for “signal-off” ECL immunoassay
6.3.2 Mimic enzyme label for “signal-off” ECL immunoassay
6.3.3 ECL resonance energy transfer for “signal-off” ECL immunoassay
6.4 "Signal-on" electrochemiluminescent immunoassay
6.4.1 Nanoemitters as labels for "signal-on" ECL immunoassay
6.4.2 Generating coreactant for "signal-on" ECL immunoassay
7 Multianalyte immunoassay
7.1 Electrode array based multianalyte immunoassay
7.2 Resolution strategies for multianalyte CL immunoassay
7.3 Chemiluminescence imaging multianalyte immunoassay
7.4 Fluorescence imaging for multianalyte immunoassay
8.1 Mass transport-accelerated immunosensing
8.2 Electric field-driven strategy for fast immunoassay
8.3 Microbubble acceleration for fast immunoassay
8.4 Infrared heating for FIIA
8.5 Micro/nano motors for fast immunoassay
9 Proximity hybridization regulated immunoassay
9.1 Proximity hybridization reaction
9.2 Ratiometric electrochemical proximity immunoassay
9.3 Proximity hybridization regulated DNA biogate
9.4 Target-driven triple-binder assembly of MNAzyme
9.5 Proximity-dependent DNAzyme for immunossay
9.6 Chemiluminescent signal switch triggered by PHR
Immunosensing for Detection of Protein Biomarkers
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