Author： Li Xinping Clarke Kimberley Li Kecheng Fougere J. Dennis Chen Aicheng
Publisher： American Scientific Publishers
ISSN： 2168-5142
Source： Journal of Bioprocess Engineering and Biorefinery, Vol.1, Iss.1, 2012-06, pp. : 77-85
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Abstract
Accelerated hydrolysis rate through the process of fiber cutting was investigated. Enzymatic hydrolysis, an important process within bioethanol production, is largely determined by cellulose accessibility within a lignocellulose substrate. SBKP and STMP fibers were examined for fiber cutting, using FQA and light microscopy. The process of fiber cutting was further studied through FE-SEM and analyzed for accelerated enzymatic hydrolysis. Fiber cutting, due to the presence of dislocation sites, was seen in SBKP and not in STMP substrate, as lignin blocked the dislocation sites in STMP fibers. There were two main pathways of fiber cutting illustrated using FE-SEM images: quickly cutting through the fiber cell wall, layer by layer; and the slower erosion through the fiber cell wall at weakened fiber areas. Images show that as the fiber cell wall was being cut, there was a significant increase in cellulose accessibility to enzymes, as microfibrils and spaces between microfibrils became exposed, and newly exposed cross-sectional area and accessible lumen. Results of this study indicate accelerated enzymatic hydrolysis may be achieved through fiber cutting by manipulating the pretreatment process, through exposing and increasing dislocation sites within the pretreatment technology design.
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