CRISPR-Cas Adaptive Immune Systems of the Sulfolobales: Unravelling Their Complexity and Diversity

By Garrett Roger A.   Shah Shiraz A.   Erdmann Susanne   Liu Guannan   Mousaei Marzieh   León-Sobrino Carlos   Peng Wenfang   Gudbergsdottir Soley   Deng Ling   Vestergaard Gisle   Peng Xu   She Qunxin  

Life, Vol. 5, Iss. 1, 2015-03 ,pp. : 783-817

MDPI

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Active and adaptive Legionella CRISPR‐Cas reveals a recurrent challenge to the pathogen

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CELLULAR MICROBIOLOGY (ELECTRONIC), Vol. 18, Iss. 10, 2016-10 ,pp. : 1319-1338

John Wiley & Sons Inc

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Genome editing technologies have been invented and improved in recent years, and there is a growing expectation that the technologies can be utilized as a tool not only for molecular biological studies, but also for genetic therapies. Among them, CRISPR‐Cas9 system that was originally found as a part of the bacterial adaptive immune system gets noticed for its easy introduction. Here, users are only required to design a guide RNA with a 20‐nt sequence that is complementary to the target DNA sequence and co‐express Cas9 with the RNA in vivo, then Cas9 protein will cleave the target DNA in a site‐specific manner. This is a portrait of a kabuki actor who plays Cas9 as indicated by the crest “ku” (‘nine’) on kimono he is wearing. The “actor” definitely has a promising future ahead of him. Designed by TRAIS Co., Ltd. (Kobe, Japan)

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GENES TO CELLS, Vol. 20, Iss. 4, 2015-04 ,pp. : i-i

John Wiley & Sons Inc

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High‐throughput screens in mammalian cells using the CRISPR‐Cas9 system

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FEBS JOURNAL (ELECTRONIC), Vol. 282, Iss. 11, 2015-06 ,pp. : 2089-2096

John Wiley & Sons Inc

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The Adaptive Immune System of Haloferax volcanii

By Maier Lisa-Katharina   Dyall-Smith Mike   Marchfelder Anita  

Life, Vol. 5, Iss. 1, 2015-02 ,pp. : 521-537

MDPI

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