Screen Printed Biosensor for Hydrogen Peroxide Based on Prussian Blue Modified Hydroxyapatite

ISSN： 1574-1443

Source： Journal of Inorganic and Organometallic Polymers and Materials, Vol.23, Iss.4, 2013-07, pp. : 917-922

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

A disposable and sensitive screen-printed carbon electrode for the determination of hydrogen peroxide (H₂O₂) was fabricated with Prussian blue-modified hydroxyapatite (PB@HAP). PB@HAP was incubated with horseradish peroxidase (HRP). The synergistic effect between HAP and PB facilitated the electron-transfer process and retained the bioactivity of HRP. The proposed biosensor effectively overcame the shortcomings of electron mediator leakage because hydroquinone was added to the base solution. The biosensor showed a low detection limit of 7 μmol/L. A linear calibration plot was obtained over the concentration range, 1-15 mmol/L; the Michaelis constant was 1.31 mmol/L, which indicated that the enzyme retained high bioactivity and excellent appetency toward H₂O₂. Additionally, the screen-printed biosensor exhibited the characteristics of strong anti-interference, high long-term stability and good reproducibility. Based on the above, PB@HAP has potential for applications in immunosensors and glucose sensors.