Author: Jia Shuang-zheng Yang Yunpeng Lang Jinghe Sun Pengran Leng Jinhua
Publisher: Oxford University Press
ISSN: 1460-2350
Source: Human Reproduction, Vol.28, Iss.2, 2013-02, pp. : 322-330
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Abstract
STUDY QUESTIONCan plasma microRNAs be used as a non-invasive diagnostic test for the detection of endometriosis?SUMMARY ANSWERPlasma miR-17-5p, miR-20a and miR-22 are down-regulated in women with endometriosis compared with those without endometriosis in mainland China.WHAT IS KNOWN ALREADYThere is currently a pressing need to develop a non-invasive diagnostic test for endometriosis. Altered circulating microRNA profiles have already been linked to various disease states.STUDY DESIGN, SIZE, AND DURATIONThis was a prospective laboratory study in a tertiary-referral university hospital in Beijing, PR China, between January 2012 and May 2012. Twenty-three women with histologically proven endometriosis and 23 endometriosis-free controls were enrolled in this study.PARTICIPANTS/MATERIALS, SETTING, AND METHODSLaparoscopic inspection of the abdominopelvic cavity was performed for each patient, and peripheral blood samples were collected before laparoscopy. Microarray-based microRNA expression profiling was used to identify differentially expressed microRNAs in plasma samples between women with and without endometriosis, and quantification of selected microRNAs was performed using quantitative RT-PCR.MAIN RESULTS AND THE ROLE OF CHANCETwenty-seven microRNAs were differentially expressed between women with and without endometriosis, of which six microRNAs (miR-15b-5p, miR-17-5p, miR-20a, miR-21, miR-22 and miR-26a) were selected for validation. MiR-17-5p, miR-20a and miR-22 were significantly down-regulated in women with endometriosis compared with controls (
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