Author: Savvaidis Ioannis Hughes Martin Poole Robert
Publisher: Springer Publishing Company
ISSN: 0003-6072
Source: Antonie van Leeuwenhoek, Vol.84, Iss.2, 2003-09, pp. : 99-107
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
The technique of differential pulse polarography is shown here to be applicable to the monitoring directly the biosorption of metal ions from solution by live bacteria from mixed metal solutions. Biosorption of Cd(II), Zn(II) and Ni(II) by P. cepacia</i> was followed using data obtained at the potential which is characteristic of the metal ion in the absence and presence of cells. Hepes buffer (pH 7.4, 50 mM) was used as a supporting electrolyte in the polarographic chamber and metal ion peaks in the presence of cells of lower amplitude were obtained due to metal-binding by the cells. Well defined polarographic peaks were obtained in experiments involving mixtures of metal ions of Cd(II)-Zn(II), Cu(II)-Zn(II), Cu(II)-Cd(II) and Cd(II)-Ni(II). Biosorption of Cd(II), Zn(II) increased with solution pH. The method was also tested as a rapid technique for assessing removal of metal ions by live bacteria and the ability of the polarographic technique in measuring biosorption of metal ions from mixed metal solutions is demonstrated. Cu(II) was preferentially bound and removal of metals was in the order Cu(II) > Ni(II) > Zn(II), Cd(II) by intact cells of P. cepacia</i>.
Related content
By Chen XinCai Shi JiYan Chen YingXu Xu XiangHua Xu ShengYou Wang YuanPeng
Canadian Journal of Microbiology, Vol. 52, Iss. 4, 2006-04 ,pp. :
By Zhang Huining Liu Li Chang Qing Wang Hongyu Yang Kai
Canadian Journal of Microbiology, Vol. 61, Iss. 6, 2015-01 ,pp. :