Molecular characterization of Mauritanian date palm cultivars using plasmid-like DNAs markers

Author: Ould Mohamed Salem A.   Rhouma S.   Zehdi S.   Marrakchi M.   Trifi M.  

Publisher: Springer Publishing Company

ISSN: 0006-3134

Source: Biologia Plantarum, Vol.51, Iss.1, 2007-03, pp. : 169-172

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Abstract

Mauritanian date palm cultivars and progenies of two controlled crosses were analyzed according to the identity of mitochondrial plasmid-like DNAs. Starting from total genomic DNA and appropriate primers, polymerase chain reaction was designed to amplify either a 373-bp or a 265-bp fragments corresponding to the S and the R-plasmid respectively. Data proved that 5 cultivars out of 10 studied have exhibited the R-plasmid suggesting their resistance to the fusariosis. The existence of intra-cultivar variability has also been revealed in the cv. Ahmar. In addition, analysis throughout progenies of two controlled crosses suggested the strict maternal transmission of the date palms’ mitochondrial genome.

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