Optimisation of UDP-glucuronyl transferase-catalysed synthesis of testosterone-β-D-glucuronide by inhibition of contaminating β-glucuronidase

Author: Stevenson D.E.  

Publisher: Springer Publishing Company

ISSN: 0951-208X

Source: Biotechnology Techniques, Vol.13, Iss.1, 1999-01, pp. : 17-21

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Abstract

Attempted synthesis of testosterone glucuronide using commercial UDP-glucuronyl transferase from either bovine or ovine sources was unsuccessful, giving 2–5% yields. This low yield was due to contaminating β-glucuronidase which hydrolysed the product. Limitation of glucuronidase activity using improved pH control and inhibitors, plus optimisation of other reaction conditions, increased the yield to 20–30% with the ovine enzyme and allowed practical preparative synthesis.