Author： Somashekar D. Venkateshwaran G. Argrawal R. Prakash M.H. Basappa S.C.
Publisher： Springer Publishing Company
ISSN： 0951-208X
Source： Biotechnology Techniques, Vol.13, Iss.1, 1999-01, pp. : 65-68
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Abstract
Among the 252 soil isolates screened, about 40% were catalase-positive. A novel soil enrichment culture technique consisting of feeding decreasing concentrations of a rich nutrient medium along with increasing concentrations of H2O2 (up to 15% v/v) in a semi- continuous glass column reactor over 15 days resulted in the isolation of high catalase producing yeasts identified as Saccharomyces cerevisiae and Schizosaccharomyces pombe. These yeasts produced 2–3 times more of intracellular catalase (1630 to 2277 U/ml) than the other microbial isolates (15 to 820 U/ml) tested. The other microorganisms gradually disappeared as the concentration of H2O2 increased in the enrichment. The technique could be used for the isolation of exclusively yeasts that might have superior industrial properties such as high ethanol, flavour, protein and lipid production.
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