Effect of Disruption Methods on the Dead-End Microfiltration Behavior of Yeast Suspension

Author: Liu Dan  

Publisher: Taylor & Francis Ltd

ISSN: 0149-6395

Source: Separation Science and Technology, Vol.45, Iss.8, 2010-01, pp. : 1042-1050

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Abstract

High voltage electrical discharges (HVED) and high pressure homogenization (HPH) were compared for extracting intracellular compounds from yeasts. The efficiency of cell disruption was evaluated by measurements of electrical conductivity, UV-spectroscopy, etc. It was shown that the HPH permits better extraction than HVED. The filtrations of yeast suspension were studied in a dead-end stirred cell. The filtration of untreated, HVED and HPH treated yeast suspensions without stirring was governed by cake formation mechanism. The filterability of HVED and especially HPH-treated yeast suspension was very low. Under stirring, different filtration mechanisms were observed for untreated and treated yeast suspensions.