Epsilon-Polylysine Fermentation and its Recovery Using Carboxymethyl Cellulose (CMC)-Conjugated Magnetite

ISSN： 0149-6395

Source： Separation Science and Technology, Vol.48, Iss.7, 2013-03, pp. : 1086-1092

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Abstract

The easily prepared polyanionic magnetic nanoparticle (MNP) was employed to recover a polycationic natural food preservative, epsilon-polylysine (-PL) from fermentation broth of Streptomyces albulus. -PL with a final concentration of 10 g/L could be produced in a 5 day pH controlled two-stage fermentation of S. albulus. The polyanionic MNP was prepared by co-precipitating Fe(II)/Fe(III) solution in the presence of water soluble carboxymethyl cellulose (CMC) at pH 11. The Langmuir isotherm can well describe the adsorption behavior between CMC-conjugated MNP (CMC-MNP) and -PL that 0.38 g of -PL could be captured by 1 g of CMC-MNP at pH 5, 37°C. Approximately, 80% of -PL adsorbed on the freshly prepared CMC-MNP could be eluted by 0.1 N HCl. The adsorption capacity of CMC-MNP remained at a constant level for at least 5 repeated adsorptions. The -PL isolated by CMC-MNP not only demonstrated an improved purity but also its antimicrobial activity as compared with α-polylysine against the bacterium E. coli.