Simultaneous analysis of three avermectins in soils by high-performance liquid chromatography with fluorescence detection

Author： Xie Xianchuan

Publisher： Taylor & Francis Ltd

ISSN： 0306-7319

Source： International Journal of Environmental and Analytical Chemistry, Vol.92, Iss.12, 2012-10, pp. : 1417-1428

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Abstract

A simple and sensitive method has been developed and validated for the determination of abamectin B_1a (ABA B_1a), emamectin B_1a (EMA B_1a) benzoate and ivermectin H₂B_1a (IVM H₂B_1a) in soils. The avermectins (AVMs) residues were extracted from soils with acetonitrile/water (9 : 1, v/v) and then were purified on C₁₈ solid-phase extraction (SPE) cartridge. After being derivatised by N-methylimidazole (N-MIM) and trifluoroacetic anhydride (TFAA), the residues of three AVMs were analysed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The method was validated in terms of system suitability, linearity, selectivity, precision, recovery, specificity and stability. There was a good linear relationship (R ²> 0.99) for three AVMs ranged from 0.01 to 5 µg mL⁻¹. The LOD and LOQs of ABA B_1a, EMA B_1a benzoate and IVM H₂B_1a for standard solutions were 1.1–1.7 and 3.6–5.7 µg L⁻¹ respectively. The accuracy of AVMs in soils was from 83.7 to 115.5% with precision less than or equal to 12.4%. Using the developed method, 9 soil samples with 9.3–12806.3 µg kg⁻¹ of AVMs residues had been detected.