Examination of the Universality of the Calibration Curve of Size Exclusion Chromatography by Using Polymers Having Complex Macromolecular Architectures

Author： Stogiou Maria Kapetanaki Cleopatra Iatrou Hermis

Publisher： Taylor & Francis Ltd

ISSN： 1023-666X

Source： International Journal of Polymer Analysis and Characterization, Vol.7, Iss.4, 2002-10, pp. : 273-283

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Abstract

The validity of the universal calibration curve (UCC) in size exclusion chromatography (SEC) was tested by using polymers having complex macromolecular architectures such as miktoarm stars (-stars) (PS)₂PI, (PS)₂PBd, (PI)₃PS, (PS-PI)₂(PI-PS)₂, (PS)(PI)(PBd), (PS)(PI)(PBd)(P4MeS), H-shaped (PI)₂PS(PI)₂, and -shaped (PS)(PI)PI(PS)(PI), as well as a model linear tetrablock copolymer of the PS-PI-PS-PI type, where PS is polystyrene, PBd polybutadiene, PI polyisoprene, and P4MeS is poly(4-methyl styrene). It was found that the universality of the relation of log(M[]) vs. peak elution volume (V_e), where M is molecular weight and [] is intrinsic viscosity, is valid even for these complex molecules. The determination of the molecular weight of a polymer by using the UCC is very sensitive to the molecular and compositional homogeneity of the sample.