Author: Ito N. Takabatake R. Seo S. Hiraga S. Mitsuhara I. Ohashi Y.
Publisher: Oxford University Press
ISSN: 0032-0781
Source: Plant and Cell Physiology, Vol.43, Iss.3, 2002-03, pp. : 266-274
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
A gene encoding a receptor-like protein kinase was isolated as the gene induced in the early period of N gene-dependent hypersensitive cell death in tobacco leaves. The kinase domain expressed as a glutathione S-transferase fusion protein was capable of autophosphorylation, indicating that this gene encodes an active protein kinase. A high level of the transcript accumulated before necrotic lesion formation in tobacco mosaic virus (TMV)-inoculated tobacco leaves carrying the N gene but it was low in a tobacco cultivar lacking the N gene. A small but reproducible increase in the transcript was found 1–2 h after a temperature shift from 30°C to 20°C even in healthy leaves, suggesting the gene expression is temperature sensitive. The gene was named WRK for wound-induced receptor-like protein kinase, because the transcript increased to a maximum within 15–30 min of wounding. In suspension cultured tobacco cells, an increase in the transcript was found 15 min after transfer to a new medium, but it was suppressed under high osmotic pressures. The wound-induced WRK accumulation was enhanced by cycloheximide treatment, but not by known defense signal compounds (salicylic acid, jasmonic acid, 1-aminocyclopropan-1-carboxylic acid and abscisic acid) and some plant hormones. Thus, WRK is a wound-inducible and temperature-sensitive protein kinase gene induced before hypersensitive cell death probably through unknown signaling pathways.
Related content
By Takabatake Reona Seo Shigemi Mitsuhara Ichiro Tsuda Shinnya Ohashi Yuko
Plant and Cell Physiology, Vol. 47, Iss. 2, 2006-02 ,pp. :
By Takabatake Reona Ando Yuko Seo Shigemi Katou Shinpei Tsuda Shinya Ohashi Yuko Mitsuhara Ichiro
Plant and Cell Physiology, Vol. 50, Iss. 6, 2009-06 ,pp. :
By Liu Jun-Jun
Tree Genetics & Genomes, Vol. 4, Iss. 4, 2008-10 ,pp. :