Uterus and endometrium: Effect of high dose progestogens on white cells and necrosis in human endometrium

ISSN： 1460-2350

Source： Human Reproduction, Vol.11, Iss.8, 1996-08, pp. : 1713-1718

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Abstract

Endometrium was collected from 20 high-dose progestogentreated patients and examined for leukocyte populations by immunohistochemistry and phloxlne-tartrazine staining. A labelled streptavidin-biotin-alkaline phosphatase technique was used with antibodies against leukocyte common antigen (LCA), T cells (CD₃) neutrophils (NP₅₇) macro phages (CD₃ KP1) and B cells (CD₃) The numbers of LCA (1070±117/mm² CD₃ (459±60/mm²), CD₆₈ (129±21/mm² positive cells and endometrial granulated lymphocytes (EGL) (236±41/mm² were significantly higher than those in the control group (P <0.001). of="" these,="" egl="" increased="" most="" (6.7="" times).="">₅₇ positive (NP₅₇₊) neutrophils were present in five out of 20 progestogen treated samples and NP₅₇₊ negative (NP₅₇₋ neutrophils in another six out of 20; while a neutrophil was only identified in one control tissue (P=0.002). Three progestogen exposed endometrial samples had either focal or extensive necrosis, and many NP₅₇₊ and NP₅₇₋ neutrophils were present in the necrotic areas. EGL, neutrophils and macro phages are known to release a number of cytolytic and cell toxic molecules which may play a role in the initiation or acceleration of progestational endometrial necrosis.