Insulin-like growth factor (IGF)-I and IGB binding protein-3 concentrations in fluid from human stimulated follicles

ISSN： 1460-2350

Source： Human Reproduction, Vol.13, Iss.2, 1998-02, pp. : 285-289

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Abstract

Insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) play an important role in regulating follicle growth and maturation. We have evaluated whether responsiveness to gonadotrophins during an in-vitro fertilization (IVF) treatment is related to follicular fluid IGF-I and IGFBP-3 concentrations. We also investigated if a difference is present in IGF-I and IGFBP-3 concentrations between patients treated with human menopausal gonadotrophin (HMG) and patients treated with highly purified follicle stimulating hormone (FSH). We have measured IGF-I and IGFBP-3 in follicular fluid from pre-ovulatory follicles in an IVF programme. All 70 patients were stimulated after being down-regulated with a gonadotrophin-releasing hormone (GnRH) analogue. IGF-I concentrations in follicular fluid were significantly inversely correlated with the number of ampoules FSH administered and number of days of FSH administration, and significantly correlated with the number of follicles aspirated. IGFBP-3 concentrations were not correlated with any other parameter measured nor were IGF-I and IGFBP-3 concentrations correlated. IGFBP-3 concentrations were significantly higher in patients receiving highly purified FSH compared with patients receiving HMG (P < 0.005).="" these="" results="" are="" new="" evidence="" that="" igf-i="" concentration="" in="" follicular="" fluid="" is="" higher="" in="" women="" who="" respond="" better="" to="" follicular="" stimulation,="" i.e.="" women="" who="" grow="" many="" follicles,="" women="" who="" need="" a="" shorter="" duration="" of="" stimulation="" and="" women="" who="" need="" fewer="" ampoules="" fsh="" before="" oocyte=""> Keywords:apoptosis/follicular fluid/folliculogenesis/IGF-I/IGFBP-3