Influence of oocyte preincubation time on fertilization after intracytoplasmic sperm injection

ISSN： 1460-2350

Source： Human Reproduction, Vol.13, Iss.8, 1998-08, pp. : 2223-2226

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Abstract

During the intracytoplasmic sperm injection (ICSI) procedure, the collected oocytes are incubated until just before ICSI. The ideal preincubation time of oocytes was investigated in 544 treatment cycles. Oocyte retrieval was carried out 35 h after human chorionic gonadotrophin administration. Oocytes were cultured for between 1 and 11 h before ICSI. Embryo transfer was performed 48h after oocyte collection. The survival, fertilization and cleavage rates of injected oocytes indicated no statistically significant differences between oocytes preincubated for different lengths of time. The proportion of good-quality embryos (grades 1 and 2) was lower at 9-11 h of preincubation time than for all the other preincubation times (P < 0.001).="" no="" statistically="" significant="" differences="" were="" detected="" in="" the="" pregnancy="" rate="" between="" each="" group="" (mean:="" 15.9%),="" although="" the="" pregnancy="" rate="" at="" 9-11="" h="" of="" preincubation="" time="" appeared="" to="" be="" low="" (7.7%).="" these="" results="" suggest="" that="" the="" oocyte="" retained="" sufficient="" potential="" for="" fertilization="" between="" 1="" and="" 9="" h="" after="" oocyte="" collection="" in="" icsi.="" for="" the="" researchers="" who="" practise="" more="" complex="" icsi="" procedures="" than="" ivf,="" it="" would="" be="" convenient="" to="" be="" able="" to="" perform="" icsi="" at="" any="" time="" between="" 1="" and="" 9="" h="" after="" oocyte=""> Keywords:delayed insemination/ICSI/incubation/oocyte