Ovary and ovulation. Colony stimulating factor-1 concentrations in blood and follicular fluid during the human menstrual cycle and ovarian stimulation: possible role in the ovulatory process

Author： Shinetugs B.

Publisher： Oxford University Press

ISSN： 1460-2350

Source： Human Reproduction, Vol.14, Iss.5, 1999-05, pp. : 1302-1306

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Abstract

To evaluate a possible role for colony stimulating factor-1 (CSF-1) in human ovarian function, the peripheral blood CSF-1 concentration throughout the human menstrual cycle and during ovarian stimulation was monitored. Blood was sampled across the menstrual cycle (n = 10) and at specific times during ovarian stimulation. In addition, the CSF-1 concentrations in follicular fluid (FF) during the follicular phase and during the luteinizing hormone (LH) surge of natural cycles, as well as 35–37 h after human chorionic gonadotrophin (HCG) during ovarian stimulation, were determined. There was no significant variation in CSF-1 concentrations during the natural menstrual cycle (median 470, range 212–1364 pg/ml). CSF-1 concentrations in FF (n = 11) were about four-fold higher (P < 0.0001)="" than="" those="" in="" plasma="" of="" the="" same="" patients.="" csf-1="" concentrations="" in="" these="" ff="" showed="" some="" stage="" dependent="" variability,="" with="" significantly="" higher="" values="" during="" the="" ovulatory="" phase="" (median="" of="" 2017="" pg/ml,="" range="" 1131–2236="" pg/ml),="" compared="" to="" mid-follicular="" phase="" (median="" 961="" pg/ml,="" range="" 830–1340="" pg/ml;="">P = 0.02). During ovarian stimulation (n = 20), the plasma concentrations were similar to a time prior to stimulation up to and including 35–37 h after HCG. On day 9 after HCG, the values (median 644, range 357–1352 pg/ml) were significantly higher compared to pre-stimulation (median 422, range 253–1598 pg/ml; P < 0.05)="" and="" 35–37="" h="" after="" hcg="" (median="" 458,="" range="" 250–658="" pg/ml;="">P < 0.01).="" ff="" concentrations="">n = 27) of CSF-1 at oocyte retrieval (median 3116, range 1824–5883 pg/ml) were about seven-fold higher than blood concentrations (median 472, range 250–1055 pg/ml; P < 0.0001).="" these="" results="" suggest="" that="" the="" intra-ovarian="" csf-1,="" possibly="" induced="" by="" lh/hcg,="" plays="" an="" important="" role="" during="" ovulation="" and="" luteinization.="">