Embryology. Establishment of a ciliated epithelial cell line from human Fallopian tube

ISSN： 1460-2350

Source： Human Reproduction, Vol.15, Iss.7, 2000-07, pp. : 1597-1603

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Abstract

Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S cells had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes and microtubules. NT/T-S cells were evaluated preliminarily on the basis of co-culture study using surplus embryos at the 4- to 8-cell stage in our IVF and embryo transfer programme. All of the 133 embryos had ≥10% fragments (based on the surface area) and were unworthy of cryopreservation. Up to 57% (16/28) of the embryos with 10–30% fragments reached the blastocyst stage by co-culture. In contrast, blastocyst formation was observed in <10% of="" the="" control="" embryos,="" some="" of="" which="" were="" co-cultured="" with="" nfl/t="" cells="" (the="" immortalized="" human="" fetal="" liver="" epithelial="" cells)="" (1/16),="" and="" the="" others="" were="" incubated="" with="" the="" co-culture="" medium="" alone="" (1/18).="" various="" cytokines/growth="" factors="" such="" as="" leukaemia="" inhibitory="" factor="" (lif),="" interleukin="" (il)-6,="" il-8="" and="" basic="" fibroblast="" growth="" factor="" were="" secreted="" by="" nt/t-s="" cells="" as="" well="" as="" by="" the="" tubal="" epithelial="" cells="" in="" primary="" culture.="" the="" establishment="" of="" a="" ciliated="" cell="" line="" will="" provide="" a="" valuable="" resource="" for="" the="" further="" studies="" of="" the="" fallopian="" tube="" in="" the="" early="" events="" of="" pregnancy.="">