Laboratory Bioassay for Sex Pheromone of the European Pine Shoot Moth, Rhyacionia buoliana

Author: DATERMAN G. E.  

Publisher: Entomological Society of America

ISSN: 1938-2901

Source: Annals of the Entomological Society of America, Vol.65, Iss.1, 1972-01, pp. : 119-123

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Abstract

Female sex pheromone of Rhyacionia buoliana (Schiffermüller) was bioassayed by observing the number of males displaying a directed upwind orientation in an airstream containing the pheromone. The incidence and type of male sexual responses depended on pheromone quantity and the length of time males were conditioned at a high illumination prior to the bioassay. A pheromone quantity equivalent to 0.005–0.01 females was necessary to induce directed orientation by males. Repeated exposure to the pheromone caused a rapid decrease in male response. Male bioassay responses indicate that, in addition and subsequent to the pheromone stimulus, a positive anemotactic stimulus and a shortrange visual stimulus may be involved in the mate-finding process.