Author： KRYSAN JAMES L. RODEN DORIS A.
Publisher： Entomological Society of America
ISSN： 1938-2901
Source： Annals of the Entomological Society of America, Vol.65, Iss.2, 1972-03, pp. : 519-519
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Abstract
Insect eggs are among the most refractory of tissues for microscopic study (Anderson 1964, Harber and Mutchmor 1970). This difficulty is probably mainly a result of the high yolk content in early stages and the impermeability of the chorion to fixatives. The yolk globules are difficult to retain because they are delicate (Telfer 1961), and there is no connective structure to hold them in place. The complications encountered in methods aimed at structural preservation alone are compounded when experimental needs also require the retention of enzyme activity. The only report of insect egg enzyme histochemistry of which we are aware is that of Salkeld (1961). She effected fixative penetration by cutting the egg into pieces; this method was adequate for her purposes because she was concerned mainly with embryonated eggs. However, it has shortcomings when one needs sections of eggs in the early stages of development.
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