Interaction of an intermediate structure of Bacillus subtilis α-amylase with alkyl-substituted sepharose 4B: A model of membrane translocation

Author： Karbalaei-Heidari Hamid Habibi Azadeh Khajeh Khosro Ranjbar Bijan Nemat-Gorgani Mohsen

Publisher： Humana Press, Inc

ISSN： 0273-2289

Source： Applied Biochemistry and Biotechnology, Vol.117, Iss.2, 2004-05, pp. : 123-132

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Abstract

An intermediate from of α-amylase from Bacillus subtilis was prepared following a previously reported procedure. Stabilization of this protein structure by various additives and its interaction with alkyl-substituted Sepharose 4B (Sepharose-lipid), used to mimic the role of the alkyl chains of the phospholipid bilayer, were investigated. Exposure of hydrophobic clusters in the protein structure on denaturation was indicated by a greater affinity of the intermediate form for interaction with the alkyl chains on the matrix, as compared with the original native structure. Near- and far-ultraviolet circular dichroism studies supported loss of tertiary structure with retention of secondary structure, as expected from molten globular intermediate forms. Based on the results presented, we suggest that interaction of a protein in its native and nonnative forms with an alkyl-substituted matrix may provide useful information regarding its capacity for insertion into and translocation across the biologic membrane.