Filter paper degrading ability of a Trichoderma strain with multinucleate conidia

Author: Toyama Hideo   Yano Makiko   Hotta Takeshi   Toyama Nobuo  

Publisher: Humana Press, Inc

ISSN: 0273-2289

Source: Applied Biochemistry and Biotechnology, Vol.137, Iss.1, 2007-12, pp. : 155-160

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Abstract

The multinucleate conidia were produced from the green mature conidia of Trichoderma reesei Rut C-30 strain by colchicine treatment. The strain with higher Filter paper degrading ability was selected among those conidia using a double layer selection medium. The selected strain, JS-2 was able to collapse the filter paper within 15 min but the original strain took 25 min to collapse it completely. Moreover, the amount of reducing sugar in the L-type glass tube of the strain, JS-2 was greater than that of the original strain. The Avicel, CMC-Na, and Salicin hydrolyzing activity of the strain, JS-2, increased 2.1 times, 1.2 times, and 3.6 times higher than that of the original strain.