Improved activity in acidic media of immobilized lysozyme

Author: Zacchigna Marina   Luca Gabriella   Lassiani Lucia   Varnavas Antonio   Pitotti Anna   Boccù Enrico  

Publisher: Humana Press, Inc

ISSN: 0273-2289

Source: Applied Biochemistry and Biotechnology, Vol.76, Iss.3, 1999-10, pp. : 171-181

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Abstract

Polyethylene glycols (PEGs) of various chain length were used tocrosslink lysozyme onto an insoluble support such as oxirane. A very high degree of modification and noinactivation of lysozyme were obtained with PEG 20000, but enzymatic activity increased up to 20 times at pH 3.0, at which point the activity of the native enzyme was lower when using Leuconos tok oenus as a macromolecular substrate.

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