Author： Thum Thomas Borlak Jürgen
Publisher： Humana Press, Inc
ISSN： 1530-7905
Source： Cardiovascular Toxicology, Vol.1, Iss.1, 2001-03, pp. : 61-72
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Abstract
Various protocols for the isolation and cultivation of adult rat cardiomyocytes were compared, and the cytoprotective potential of the reversible myosin ATPase inhibitor butanedione monoxime (BDM) was evaluated based on cell yield, cell vitality, lactate dehydrogenase (LDH) and creatine kinase (CK) release, and the mRNA expression of atrial natriuretic peptide (ANP). Overall, a yield of 11.9×106 cells with >92% cell vitality was obtained when BDM was added to the isolation and cultivation buffers. In contrast, cell vitality ranged from 30% to 70% and cell yield was (4–10)×106 when standard methods for the isolation of cardiomyocytes were used. Butanedione monoxime, at a 15 m
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