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Characterization of the causal agent of poplar anthracnose occurring in the Beijing region

Author: Li Zheng   Liang Ying Mei   Tian Cheng Ming  

Publisher: Mycotaxon

ISSN: 2154-8889

Source: Mycotaxon, Vol.120, Iss.1, 2012-09, pp. : 277-286

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Abstract

Twenty fungal isolates derived from infected poplar leaves collected in four Beijing regional districts during 2009 and 2010 were examined for morphological and cultural characteristics. Multi-gene phylogenetic sequence analyses of the rDNA ITS (ITS1-5.8S-ITS2) region and the β-tubulin2, partial actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GPDH), and glutamine synthetase (GS) genes were performed for these isolates. The morphological and cultural evaluations and DNA sequence analyses demonstrated that 16 isolates represented Colletotrichum gloeosporioides while the remaining four isolates, all from Shi Jingshan district, represent a new species, Colletotrichum populi, which is described and illustrated.

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