Isozyme Specificity During Germination and Early Growth of Knobcone Pine

Publisher： Society of American Foresters

ISSN： 0015-749X

Source： Forest Science, Vol.17, Iss.4, 1971-12, pp. : 494-498

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Abstract

Five enzyme classes from 11 developmental stages of germinating embryos were separated by starch gel electrophoresis. Alcohol dehydrogenase isozymes found in embryos of dry seed were most active at the time of radicle emergence; activity decreased thereafter, fading below the level of detection when seed coats were shed. Peroxidase isozymes were absent and esterase isozymes were nearly absent from stratified seed, but both increased steadily in number and stainability from time of radicle emergence through epicotyl elongation. Leucine aminopeptidase and catalase isozymes were detectable in all growth stages. In later stages, esterases and leucine aminopepfidases were found primarily in seedling tops, and many catalase and peroxidase isozymes differed between roots and tops. Both the developmental stage of the plant and the tissue assayed determine the presence of specific isozymes. Forest Sci. 17: 494-498.